mouse ngf β elisa kit  (Boster Bio)

Journal: Molecular neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood-Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats.

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: Fig. 2 The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A, B, C, D, E, F The NGF + SMES group had signifi- cant effect except PrL and MS. The results are demonstrated as the mean ± SEM (n = 5, each group).*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are dem- onstrated as the mean ± SEM (n = 5, each group). **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. hippocampus group. a, b, c, d, e, f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM (n = 5 or 8, each group). *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. NGF + SMES group. g The hip- pocampus had significant effect. The results are demonstrated as the mean ± SEM (n = 6, each group). ****P < 0.0001 vs. hip- pocampus group

Article Snippet: Enzyme‐Linked Immunosorbent Assay Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse BetaNGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: Experimental framework. Experiment 1: The study divided rats into control and MCAO/R groups to test memory impairment after modeling. The control group received no intervention, while the MCAO/R group was modeled using the suture method and tested with water maze or object recognition experiments 28 days later. Experiment 2: The study tested the continuous therapeutic effect by dividing MCAO/R rats into different groups and using normal rats as a control. After treatment, a water maze experiment was conducted for 6 days, followed by detection of IF, IHC, and TEM. Experiment 3: MCAO/R rats were divided into different groups for ELISA testing to verify the permeability of mNGF in various brain regions by SMES. Another set of MCAO/R rats were divided into groups for WB and ICH verification of the p65-VEGFA-TJs signal axis to explore the effect of SMES on opening the BBB of the hippocampus

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Permeability

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A , B , C , D , E , F The NGF + SMES group had significant effect except PrL and MS. The results are demonstrated as the mean ± SEM ( n = 5, each group). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 5, each group). ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. hippocampus group. a , b , c , d , e , f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM ( n = 5 or 8, each group). * P < 0.05, ** P < 0.01, and **** P < 0.0001 vs. NGF + SMES group. g The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 6, each group). **** P < 0.0001 vs. hippocampus group

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Senescence of endplate osteoclasts induces sensory innervation and spinal pain

doi: 10.7554/eLife.92889

Figure Lengend Snippet: ( a ) Enzyme-linked immunosorbent assay (ELISA) analysis showing the concentration of Netrin-1 in L3–5 endplates of adult sham, lumbar spine instability (LSI) + PBS, and LSI + ABT263 mice. ( b ) ELISA analysis showing the concentration of NGF in L3–5 endplates of adult sham, LSI + PBS, and LSI + ABT263 mice. n=3 per group. Statistical significance was determined by one-way ANOVA, and all data are shown as means ± standard deviations.

Article Snippet: We determined the concentration of Netrin-1 (LSBio, LS-F5882) and NGF (Boster, EK0470) in the L3–L5 endplates using the ELISA Development Kit according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay