mouse ngf β elisa kit  (Boster Bio)

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: Experimental framework. Experiment 1: The study divided rats into control and MCAO/R groups to test memory impairment after modeling. The control group received no intervention, while the MCAO/R group was modeled using the suture method and tested with water maze or object recognition experiments 28 days later. Experiment 2: The study tested the continuous therapeutic effect by dividing MCAO/R rats into different groups and using normal rats as a control. After treatment, a water maze experiment was conducted for 6 days, followed by detection of IF, IHC, and TEM. Experiment 3: MCAO/R rats were divided into different groups for ELISA testing to verify the permeability of mNGF in various brain regions by SMES. Another set of MCAO/R rats were divided into groups for WB and ICH verification of the p65-VEGFA-TJs signal axis to explore the effect of SMES on opening the BBB of the hippocampus

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Permeability

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A , B , C , D , E , F The NGF + SMES group had significant effect except PrL and MS. The results are demonstrated as the mean ± SEM ( n = 5, each group). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 5, each group). ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. hippocampus group. a , b , c , d , e , f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM ( n = 5 or 8, each group). * P < 0.05, ** P < 0.01, and **** P < 0.0001 vs. NGF + SMES group. g The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 6, each group). **** P < 0.0001 vs. hippocampus group

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Senescence of endplate osteoclasts induces sensory innervation and spinal pain

doi: 10.7554/eLife.92889

Figure Lengend Snippet: ( a ) Enzyme-linked immunosorbent assay (ELISA) analysis showing the concentration of Netrin-1 in L3–5 endplates of adult sham, lumbar spine instability (LSI) + PBS, and LSI + ABT263 mice. ( b ) ELISA analysis showing the concentration of NGF in L3–5 endplates of adult sham, LSI + PBS, and LSI + ABT263 mice. n=3 per group. Statistical significance was determined by one-way ANOVA, and all data are shown as means ± standard deviations.

Article Snippet: We determined the concentration of Netrin-1 (LSBio, LS-F5882) and NGF (Boster, EK0470) in the L3–L5 endplates using the ELISA Development Kit according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Biomedicines

Article Title: Ribosome Biogenesis Serves as a Therapeutic Target for Treating Endometriosis and the Associated Complications

doi: 10.3390/biomedicines10010185

Figure Lengend Snippet: Regulation of nerve fiber growth in endometriotic lesions of mice with induced endometriosis. ( a ) The protein levels of NGF ( left ) and IGF-1 ( right ) in the peritoneal fluid were analyzed by sandwich ELISA. The data were normalized with the average levels in mice treated with vehicle ( n = 15 for each group). ( b ) Immunofluorescence staining was performed to detect the presence of nerve fibers (PGP9.5 + ) in endometriotic lesions from different groups ( left ). The fluorescence intensity was averaged by using data from 10 independent tissue sections ( right ). Statistical differences between vehicle and drug-treated groups were compared by t -test. The p values were presented as *: p value < 0.05, **: p value < 0.01, and ***: p value < 0.001.

Article Snippet: The ELISA kits were purchased from Thermo Fisher Scientific (EM9RB for mouse β-NGF and EMIGF1 for mouse IGF-1, Waltham, MA, USA).

Techniques: Sandwich ELISA, Immunofluorescence, Staining, Fluorescence